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Image gallery

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	Selected lysosomal markers in human fibroblasts Human fibroblasts stained for cathepsin D (top left), beta glucocerebrosidase (top right), DAPI nuclear staining (bottom left) and merged image (bottom right). Plate was prepared in ImageJ sft.
	Fibrinogen deposits in two different cases of fibrinogen storage disease (liver tissue) Fibrinogen (green), protein disulfide isomerase (PDI) - endoplasmic reticulum marker (red), DAPI nuclear stain (blue). Rightmost images represent colocalization maps of red/green channels using overlap coefficient (0-1) scaled with appropriate LUT (calculated in Huygens sft). Plate was prepared in ImageJ sft.
	3D rendered image of glial phagocyte (violet) and astrocyte (green). Reconstruction performed in Imaris sft.
	Tobacco expressing GFP in endoplasmic reticulum, orthogonal view (sample is courtesy of Dr.Petrasek, Institute of Experimental Botany Academy of Sciences, Czech Republic).
	Tobacco expressing GFP in endoplasmic reticulum labeled with FM 1-43 (Molecular Probes - Invitrogen) for cell membrane. Image acquired in the spectral mode (left) of Nikon C1si. Right panel corresponds to spectrally unmixed image. Plate was prepared in ImageJ sft.
	Att20 cells with DAPI labeled nuclei and FM 1-43 labeled cytoplasmic membrane (Molecular Probes). Right side of the image was deconvolved with Huygens Professional software (SVI, Hilversum, The Netherlands)
	Actin cytoskeleton component (green), vinculin - adherent junctions (red), nucleus - blue. Z-stack image rendered in Imaris Personal.
	Secretory granules in cultured cells. Effect of deconvolution on wide-field volume images, the image acquisition was done on the TE2000 inverted microscope equipped with Vosskuhler CCD-1300 camera. The deconvolution was performed in Huygens Professional sft, image rendering was done in Imaris Personal. A,C - original data; B,D - deconvolved images.