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Technical specifications of TE2000E C1si laser scanning confocal microscope



TE2000E (motorized modules)
  •   nosepiece
  •   condensers
  •   BP filter turret
  •   Z - drive
  •   analyzer
  •   epifluorescence shutter
  •   light-path (5 ports)
  •   diascopic illumination
  •   X-Cite
Objectives

Apo TIRF 60x N.A. 1.49
Plan Fluor 40x N.A.1.3
Plan Fluor 10x N.A. 0.3

Condensers

Abbe
DIC N2 (Nomarski optics)

BP filters

UV-2A Ex.330-380/ DM 400/Em.420LP
FITC Ex.465-495/DM505/Em.515-555
TRITC Ex.528-553/DM565/Em.578-633

CCD

Vosskuhler CCD-1300 camera		 Laser scanning confocal microscope C1si

Laserlines

405nm diode, 488nm, 514nm, 543nm (AOM intensity tuning)
633nm (ND4/8 tuning)

Dichroic mirrors

488/543/633nm
405/488/543nm
20/80, 10/90 (reflectance/transmittance) beamsplitters for spectral detection

PMT band pass filtering setup

Filter set 1 515+/-15nm; 590+/-15nm; 650nm + LP
Filter set 2 450+/-15nm; 515+/-15nm; 610nm+/-37,5nm

Spectral detection C1si

32 channels
400-750 nm detection range
2.5/5/10 nm discrimination
Linear unmixing algorithm based on single value decomposition method

LiveScan Swept field confocal system (SFC)

Laserlines

488nm (Sapphire 488-50), 561nm (Jive-50), 643nm (RCL-035-643) (AOM intensity tuning)

Detector

Nikon DQC-FS EM-CCD camera

System maximum scanning speed

250 fps (slit mode)
100 fps (pinhole mode)

 Perfect focus system (PFS)


Technical specifications of Nikon E400 - widefield epifluorescent microscope



Objectives

Plan 10x/0.25
Plan 20x/0.40
Plan 40x/0.65
60x/0.80
Plan 100x/1.25 oil 		BP filters

DAPI/Hoechst/AMCA/C.blue Ex.325-375/DM400/Em.435-485
B-2A Ex.450-490/DM505/Em.520LP
G-2A Ex.510-560/575/Em.590LP

CCD DS-U1