Marie Hubálek Kalbáčová
Team leader - Assistant professor (2007- 2014)
Associated professor(2014-present)
Contact: mkalb@lf1.cuni.cz
Research Focus
At present I use different nanoscale materials (carbon nanotubes, nanocrystalline diamonds, titanium alloys) to grow osteoblasts and plan to start growing mesenchymal stem cells on them as well to find the most suitable osteoconductive nanosurface useful for new implant coatings in orthopedics. I am interested in osteoconductivity of differently functionalized materials focusing on adhesion, metabolic activity and gene expression. I have also started project of photodynamic therapy for cancer cells using UV illuminated titanium nanotubes.
Curriculum Vitae
1993-1996 Bc. in Biology , Faculty of Natural Sciences, Charles University, Prague, Czech Republic
1996-1998 M.Sc. in Biology , Faculty of Natural Sciences, Charles University, Prague, Czech Republic
1998-2003 Ph.D. in Molecular Genetics, Faculty of Natural Sciences, Charles University, Prague, Czech Republic
2003-2004 Postdoctoral Research Fellow, Institute of Inherited Metabolic Disorders, 1st Medical Faculty, Charles University, Prague, Czech Republic
2004-2006 Postdoctoral Research Fellow, Institute of Physiological Chemistry, Medical Faculty of University of Technology, Dresden, Germany
2007- Assistant professor, Institute of Inherited Metabolic Disorders, 1st Medical Faculty, Charles University, Prague, Czech Republic
Publiciations
2016
Verdanova M., Rezek B., Broz A., Ukraintsev E., Babchenko O., Artemenko A., Izak T., Kromka A., Kalbac M., Hubalek Kalbacova M., Nanocarbon Allotropes - Graphene and Nanocrystalline Diamond - Promote Cell Proliferation, in press in Small, 12, p.2499-2509, 2016, IF 8,368
Ostrovska L., Vistejnova L., Dzugan J., Slama P., Kubina T., Ukraintsev E., Kubies D., Kralickova M. , Hubalek Kalbacova M., Biological evaluation of ultra-fine titanium with improved mechanical strength for dental implant engineering, in press in Journal of Materials Science, 51, p. 3097-3110, 2016 – IF 2,371
2015
Suchy T., Supova M., Sauerova M., Verdánová M., Sucharda Z., Ryglova S., Zaloudkova M, Sedlacek R., Hubalek Kalbacova M., The effects of different cross-linking conditions on collagen-based nanocomposite scaffolds - an in vitro evaluation using mesenchymal stem cells, Biomedical Materials,10: 065008, 2015 – IF 3,697
Ukraintsev E, Broz A., Hubalek Kalbacova M., Kromka A., Rezek B., Stochasitc model explains formation of cell arrays on H/O diamond patterns, Biointerphases, 10 (4): 041006-1-9, 2015 – IF 3,374
Sauerova P., Verdanova M., Mravec F., Pilgrova T., Venerova T., Hubalek Kalbacova M., Pekar M..: Hyaluronic acid, a useful modulator of the cytotoxic effects of cationic surfactants, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 493: 155-161, 2015 – IF 2,752
2014
Verdanova M., Pytlik R., Hubalek Kalbacova M., Evaluation of sericin as an FBS-replacing cryoprotectant during freezing of human mesenchymal stromal cells and human osteoblast-like cells, Biopreservation and Biobanking, 12: 99-105, 2014 – IF 1,5
Fucikova A., Valenta J, Pelant M., Hubalek Kalbacova M., Broz A., Rezek B., Kromka A, Bakaeva Z.: Silicon nanocrystals and nanodiamonds in live cells: photoluminescence characteristics, cytotoxicity and interaction with cell cytoskeleton, RSC Advances, 4: 10334-10342, 2014 – IF 2,562
Hubalek Kalbacova M., Verdanova M., Broz A., Vetushka A, Fejfar A., Kalbac M.: Modulated surface of single-layer graphene controls cell behaviour, Carbon, 72: 207-214, 2014 – IF 5,868
Kalbacova M., Verdanova M., Mravec F., et al.: Effect of CTAB and CTAB in the presence of hyaluronan on selected human cell types, in press in Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2014 – IF 2,108
2012
Kalbacova M., Broz A., Kalbac M.: Influence of the fetal bovine serum proteins on the growth of human osteoblast cells on graphene, J Biomed Mater Res Part A, 2012 - IF 2,625
Kratka M., Kromka A., Ukraintsev E., Ledinsky M., Broz A., Kalbacova M., Rezek B.: Function of thin film nanocrystallihne diamond-protein SGFET independent of grain size, Sensors and Actuators B, 166-167, 239-245, 2012 - IF 3,898
Neykova N. Broz A., Remes Z., Hruska K., Kalbacova M., Kromka, A; Vanecek,M: ZnO hedgehog-like structures for control cell cultivation , Applied Surface Science 258, 3485-3489, 2012 - IF 2,103
2011
Kalbacova M., Broz A., Kromka A., Babchenko O., Kalbac M.: Controlled oxygen plasma treatment of single-walled carbon nanotube films improves osteoblast cells attachement and enhances their proliferation , CARBON Volume: 49,2926-2934, 2011 – IF 5,378
2010
Grünfeld J-P., Hwu WL., Chien YH., Lee NC., Chiang SC., Dobrovolny R., Huang AC.,
Yeh HY., Chao MC., Lin SJ., Kitagawa T., Desnick RJ., Hsu LW., Van Keimpema L.,
Nevens F., Vanslembrouck R., Van Oijen GH., Hoffmann AL., Dekker HM., De Man RA.,
Drenth JPH., Alamovitch S., Plaisier E., Favrole P., Prost C., Chen Z., Van Agrmael T.,
Marro P., Ronco P., Zivna M., Hulkova H., Matignon M., Hodanova K., Vylet'al P., Kalbacova M.,
Baresova V., Sikora J., Blazkova H., Zivny J., Ivanek R., Stranecky V., Sovova J., Claes K., Lerut E.,
Fryns JP., Hart PS., Hart TC., Adams JN., Pawtowski A., Clemessy M., Gasc JM., Gubler MC., Antignac C.,
Elleder M., Kapp K., Grimbert P., Bleyer AK., Kmoch S., Brown EJ., Schlöndorff JS., Becker DJ.,
Tsukaguchi H., Uschinski AL., Higgs HN., Henderson JM., Pollak MR., More on clinical renal genetics , Clin J Am Soc Neph , 5, 563-567, 2010
Kalbacova M., Broz A., Kong J., Kalbac M., Graphene substrates promote adherence of human osteolbasts and mesenchymal stromal cells , Carbon 48,4323-4329, 2010 – IF 4,504
Rezek B., Kratka M., Kromka A., Kalbacova M., Effects of protein inter-layers on cell-diamond FET characteristics , Biosensors and Bioelectronics, 26,1307-1312, 2010 – IF 5,361
Jin H., Heller DA., Kalbacova M., Kim J-H., Zhang J., Boghossian AA., Maheshri N., Strano MS., Detection of single-molecule H2O2 singaling from epidermal growth factor receptor using fluorescent single-walled carbon nanotubes , Nature Nanotechnology , 5, 302-309, 2010 – IF 30,306
Rezek B., Ukraintsev E., Kromka A., Ledinsky M, Broz A., Noskova L., Hartmannova H., Kalbacova M., Assembly of osteoblastic cell micro-arrays on diamond guided by protein pre-adsorption , Diam Rel Mater 19:153-157, 2010 – IF 1,825
Hempel U., Hefti T., Kalbacova M., Wolf-Brandstetter C., Dieter P., Schlottig F., Response of osteoblast-like SAOS-2 cells to zirconia ceramics with different surface topographies , Clin Oral Implant Res, 21:171-181, 2010 – IF 2,92
2009
Kalbacova M., Rezek B., Baresova V., Wolf-Brandstetter C., Kromka A., Nanoscale topography of nanocrystalline diamonds promotes differentiation of osteoblasts , Acta Biomaterialia 5:3076-3085, 2009 - IF 3,
Kalbacova M., Broz A., Babchenko O., Kromka A., Study on cellular adhesion of human osteoblasts on nano-structured diamond films , Physica Status Solidi B , 246: 2774-2777, 2009 – IF 1,15
Ukraintsev E., Rezek B., Broz A., Kalbacova M., Long-term adsorption of fetal bovine serum on H/O-terminated diamond studied in situ by atomic force microscopy , Physica Status Solidi B , 246: 2832-2835, 2009 – IF 1,15
Babchenko O., Kromka A., Hruska K., Kalbacova M., Broz A., Vanecek M., Fabrication of nano-structured diamond films for SAOS-2 cell cultivation , Physica Status Solidi A, 206: 2033-2037, 2009 – IF 1,228
Broz A., Baresova V., Kromka A., Rezek B., Kalbacova M., Strong influence of hierarchically structured diamond nanotopography on adhesion of human osteoblasts and mesenchymal cells , Physica Status Solidi A, 206: 2038-2041, 2009 – IF 1,228
Michalikova L., Rezek b., Kromka A., Kalbacova M., CVD diamod films with hydrophilic micro-patterns for self-organisation of human osteoblasts , Vacuum 84: 61-64, 2009 – IF 0,975
Zivna M., Hulkova H., Matignon M., Hodanova K., Vyletal P., Kalbacova M., Baresova V., Sikora J., et al., Dominant Renin Gene Mutations Associated with Early-Onset Hyperuricemia, Anemia, and Chronic Kidney Failure , Am J Hum Gen 85(2) :204-213, 2009 – IF 12,303
Pytlik R., Stehlik D., Soukup T., Kalbacova M., Rypacek F., Trc T., Mulinkova K., Michnova P., Kideryova L., Zivny J., et al., The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering , Biomaterials 30 : 3415-3427, 2009 – IF 7,365
Rezek B., Michalikova L., Ukraintsev E., Kromka A., Kalbacova M., Micro-Pattern guided adhesion of osteoblasts on diamond surfaces , Sensors 9 (5):3549-3562, 2009 – IF 1,821
Kromka A., Rezek B., Kalbacova M., Baresova V., Zemek J., Konak C., Vanecek M., Diamond seeding and growth of hierarchically structured films for tissue engineering , Adv. Eng. Mat. 11:B71-B76, 2009 – IF 1,761
Rezek B., Ukraintsev E., Michalikova L., Kromka A., Zemek J., Kalbacova M., Adsorption of fetal bovine serum on H/O-terminated diamond studied by atomic force microscopy , Diam Rel Mater 18(5-8):918-922, 2009 - IF 1,822
2008
Kalbacova M., Michalikova L., Baresova V., Kromka A., Rezek B., Kmoch S., Adhesion of osteoblasts on chemically patterned nanocrystalline diamonds , Physica Status Solidi B 245(10): 2124-2127, 2008 - IF 1,166
Kalbacova M., Macak J.M., Schmidt-Stein F., Mierke C. T., Fabry B., Schmuki P., TiO2 nanotubes: Photocatalyst for cancer cell killing , Physica Status Solidi (RRL) 2: 194-196, 2008 - IF 2,147
2007
Kalbacova M., Kalbac M., Dunsch L., Kromka A., Vanecek M., Rezek B., Hempel U., Kmoch S., The effect of SWCNT and nano-diamond films on human osteoblast cells , Physica Status Solidi B 244 (11): 4356-4359, 2007 – IF 1,071
Kalbacova M., Kalbac M., Dunsch L., Hempel U., Influence of single-walled carbon nanotube films on metabolic activity and adherence of human osteoblasts , Carbon 45:2266-2272, 2007 – IF 4,26
Kalbacova M., Roessler S., Hempel U., et al., The effect of electrochemically simulated titanium cathodic corrosion products on ROS production and metabolic activity of osteoblasts and monocytes/macrophages, Biomaterials 28 (22): 3263-3272, 2007 – IF 6,262
Tsaryk R., Kalbacova M., Hempel U., et al., Response of human endothelial cells to oxidative stress on Ti6Al4V alloy , Biomaterials 28 (5): 806-813, 2007 – IF 6,262
2006
Kalbacova M., Kalbac M., Dunsch L., et al., The study of the interaction of human mesenchymal stem cells and monocytes/macrophages with single-walled carbon nanotube films , Physica Status Solidi B-Basic Solid State Physics 243 (13): 3514-3518, 2006 – IF 0,967
Book chapters
B. Rezek, M. Krátká, E. Ukraintsev, O. Babchenko, A. Kromka, A. Brož, M. Kalbacova: Diamond as functional material for bioelectronics and biotechnology In: “New Perspectives in Biosensors Technology and Applications” Intech, pp. 177-196, ISBN 978-953-307-448-1, 2011.
Hubálek Kalbáčová M. Transport Of Biomolecules Into Cells. In Press. In D. Smejkalova, &
K. Nesporova, Nanoparticulate drug delivery systems (pp. 99-132). ISBN 978-80-260-
7611-7