Ústav patologické fyziologie 1. LF UK

Laboratoř pro molekulární patofyziologii a molekulární morfologii

Řešitel: prof. MUDr. Emanuel Nečas, DrSc.



Články v odborných časopisech s impakt faktorem:

Rok vydání Název článku Autoři Bibliografické údaje Impakt faktor Poznámky
2005 Hepcidin: a direct link between iron metabolism and immunity. Vyoral D, Petrak J. Int J Biochem Cell Biol. 2005 Sep;37(9):1768-73 -  
2006 Evaluation of mitochondrial membrane potential using a computerized device with a tetraphenylphosphonium-selective electrode Labajova A, Vojtiskova A, Krivakova P, Kofranek J, Drahota Z, Houstek J. Anal Biochem. 2006 Jun 1;353(1):37-42. -  
2006 Hepcidin mRNA levels in mouse liver respond to inhibition of erythropoiesis. Vokurka M, Krijt J, Sulc K, Necas E. Physiol Res. 2006;55(6):667-74 -  
2006 Effect of Lipopolysaccharide and Bleeding on the Expression of Intestinal Proteins Involved in Iron and Haem Transport Krijt J, Vokurka M, Sefc L, Duricova D, Necas E. Folia Biol (Praha). 2006;52(1-2):1-5 -  
2006 High levels of circulating CD34 cells, dacrocytes, clonal hematopoiesis, and JAK2 mutation differentiate myelofibrosis with myeloid metaplasia from secondary myelofibrosis associated with pulmonary hypertension. Popat U, Frost A, Liu E, Guan Y, Durette A, Reddy V, Prchal JT Blood. 2006;107(9):3486-8 -  
2006 DMT1 mutation: response of anemia to darbepoetin administration and implications for iron homeostasis Pospisilova D, Mims MP, Nemeth E, Ganz T, Prchal JT Blood. 2006;108(1):404-5 -  
2006 Hypoxia- inducible Factor-1 Deficiency Results in Dysregulated Erythropoiesis Signaling and Iron Homeostasis in Mouse Development Yoon D, Pastore YD, Divoký V, Liu E, Mlodnická AE, Rainey K, Poňka P, Semenza GL, Schumacher A, Prchal JT J Biol Chem. 2006;281(35):25703-11 -  
2006 Tetraphenylphosphonium-selective electrode as a tool for evaluating mitochondrial permeability transition pore function in isolated rat hepatocytes. Labajova A, Kofranek J, Krivakova P, Cervinkova Z, Drahota Z Gen Physiol Biophys. 2006 Sep;25(3):325-31 -  
2006 Evaluation of mitochondrial membrane potential using a computerized device with a tetraphenylphosphonium-selective electrode. Labajova A, Vojtiskova A, Krivakova P, Kofranek J, Drahota Z, Houstek J Anal Biochem. 2006 Jun 1;353(1):37-42 -  
2006 Magnetic IDA-modified hydrophilic methacrylate-based polymer microspheres for IMAC protein separation. Prikryl P, Horak D, Ticha M, Kucerova Z J Sep Sci. 2006 Nov;29(16):2541-9 -  
2006 Immunoaffinity carrier prepared by immobilization of antibody via Co3+-chelate. Zatloukalova E, Kucerova Z J Sep Sci. 2006;29(8):1082-7 -  
2006 Alterations of uromodulin biology: a common denominator of the genetically heterogeneous FJHN/MCKD syndrome. Vylet'al P, Kublova M, Kalbacova M, Hodanova K, Baresova V, Stiburkova B, Sikora J, Hulkova H, Zivny J, Majewski J, Simmonds A, Fryns JP, Venkat-Raman G, Elleder M, Kmoch S Kidney Int. 2006 Sep;70(6):1155-69 -  
2006 Cell death signalling pathways in the pathogenesis and therapy of haematologic malignancies: overview of therapeutic approaches. Klener P Jr, Andera L, Klener P, Necas E, Zivny J Folia Biol (Praha). 2006;52(4):119-36 -  
2006 Protein kinases, their function and implication in cancer and other diseases. I. SHCHEMELININ, L. ŠEFC, E. NEČAS Folia Biol (Praha). 2006;52(3):81-101 -  
2006 Protein Kinase Inhibitors I. Shchemelinin, L. Sefc, E. Necas Folia Biol (Praha). 2006;52(4):137-148 -  
2007 Imatinib effect on growth and signal transduction in polycythemia vera. Gaikwad A, Verstovsek S, Yoon D, Chang KT, Manshouri T, Nussenzveig R, Cortes J, Vainchenker W, Prchal JT Exp Hematol. 2007 Jun;35(6):931-8 3,147  
2007 Native proteomic analysis of protein complexes in murine intestinal brush border membranes. Babusiak M, Man P, Petrak J, Vyoral D Proteomics. 2007 Jan;7(1):121-9 5,479  
2007 In vitro expansion of erythroid progenitors from polycythemia vera patients leads to decrease in JAK2 V617F allele. Gaikwad A, Nussenzveig R, Liu E, Gottshalk S, Chang K, Prchal JT Exp Hematol. 2007 Apr;35(4):587-95 3,147  
2007 Risedronate has no adverse effects on mouse haematopoiesis. Sefc L, Broulík P, Pelichovská T, Necas E Folia Biol (Praha). 2007;53(4):143-5 0,596  
2007 Pattern storage in a sparsely coded neural network with cyclic activation. Stroffek J, Kuriscak E, Marsalek P Biosystems. 2007 May-Jun;89(1-3):257-63 1,646  
2007 Iron-independent specific protein expression pattern in the liver of HFE-deficient mice. Petrak J, Myslivcova D, Halada P, Cmejla R, Cmejlova J, Vyoral D, Vulpe CD Int J Biochem Cell Biol. 2007;39(5):1006-15 4,009  
2007 Hypoxia-inducible factor-1 deficiency results in dysregulated erythropoiesis signaling and iron homeostasis in mouse development. Yoon D, Pastore YD, Divoky V, Liu E, Mlodnicka AE, Rainey K, Ponka P, Semenza GL, Schumacher A, Prchal JT J Biol Chem. 2006 Sep 1;281(35):25703-11 5,581  
2007 Effect of phlebotomy on hepcidin expression in hemojuvelin-mutant mice. Krijt J, Niederkofler V, Salie R, Sefc L, Pelichovská T, Vokurka M, Necas E Blood Cells Mol Dis. 2007 Jul-Aug;39(1):92-5 2,555  
2007 Proteomic analysis of erythroid differentiation induced by hexamethylene bisacetamide in murine erythroleukemia cells. Petrak J, Myslivcova D, Man P, Cmejlova J, Cmejla R, Vyoral D Exp Hematol. 2007 Feb;35(2):193-202 3,147  
2007 Proteomic analysis of hepatic iron overload in mice suggests dysregulation of urea cycle, impairment of fatty acid oxidation, and changes in the methylation cycle. Petrak J, Myslivcova D, Man P, Cmejla R, Cmejlova J, Vyoral D, Elleder M, Vulpe CD Am J Physiol Gastrointest Liver Physiol. 2007 Jun;292(6):G1490-8 3,761  
2007 Mitochondrial complex I inhibition in cerebral cortex of immature rats following homocysteic acid-induced seizures. Folbergrová J, Jesina P, Drahota Z, Lisý V, Haugvicová R, Vojtísková A, Houstěk J Exp Neurol. 2007 Apr;204(2):597-609 4.156  
2008 Chromatin remodeling and SWI/SNF2 factors in human disease. Kokavec J, Podskocova J, Zavadil J, Stopka T Front Biosci. 2008 May 1;13:6126-34 2,989  
2008 Immobilized Metal Affinity Chromatography of Phosphorylated Proteins Using High Performance Sorbents Lenka Novotna, Martin Hruby, Milan J. Benes and Zdenka Kucerova Chromatographia 2008, 68(5/6): 381-386 1,145  
2008 Interaction of pepsin with aromatic amino acids and their derivatives immobilized to Sepharose. Frýdlová J, Kucerová Z, Tichá M J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Feb 15;863(1):135-40 2,935  
2008 Optimization of Affinity Chromatography of Pepsins on Iodinated l -Tyrosine-Sepharose Jana Frýdlová, Marie Tichá and Zdenka Kučerová Chromatographia 2008 May; 67(Suppl.1): 41 - 45 1,145  
2009 TRAIL-induced apoptosis of HL60 leukemia cells: Two distinct phenotypes of acquired TRAIL resistance that are accompanied with resistance to TNFα but not to idarubicin and cytarabine Pavel Klener Jr., Sergiu Leahomschi, Jan Molinsky, Tereza Simonova, Emanuel Necas, Zdenka Gasova, Jaroslav Cermak, Ludmila Dolezalova, Ladislav Andera and Jan Zivny Blood Cells Mol Dis 2009; 42(1): 77 - 84 -  
2009 Identification of mutations in the ribosomal protein L5 (RPL5) and ribosomal protein L11 (RPL11) genes in Czech patients with Diamond-Blackfan anemia. Cmejla R, Cmejlova J, Handrkova H, Petrak J, Petrtylova K, Mihal V, Stary J, Cerna Z, Jabali Y, Pospisilova D Hum Mutat. 2009 Mar;30(3):321-7 -  
2009 Nuclear localization of ISWI ATPase Smarca5 (Snf2h) in mouse. Vargova J, Vargova K, Skoultchi AI, Stopka T Front Biosci (Elite Ed). 2009 Jun 1;1:553-9 -  
2009 SWI/SNF2 proteins in germinal and early embryonic development Kokavec Juraj,Vargova Jarmila, Burda Pavel,Vargova Karin, Necas Emanuel, Stopka Tomas Folia Zool 58 Suppl.1, 2009,p.5-16 -  
2009 Multiple roles of secretory lipocalins (Mup,Obp) in mice Stopkova Romana, Hladovcova Denisa, Kokavec Juraj, Vyoral Daniel, Stopka Pavel Folia Zool 58 Suppl.1, p.31-42 -  
2009 PU.1 activation relieves GATA-1-mediated repression of Cebpa and Cbfb during leukemia differentiation. Burda P, Curik N, Kokavec J, Basova P, Mikulenkova D, Skoultchi AI, Zavadil J, Stopka T Mol Cancer Res. 2009 Oct;7(10):1693-703 -  
2009 Immobilization of α - chymotrypsin to magnetic particles and their use for proteolytic cleavage of porcine pepsin A Sustrova, Barbora;Novotna, Lenka;Kucerova, Zdenka; Ticha, Marie Journal of Molecular Catalysis B: Enzymatic 60,p.22-28, 2009 -  
2009 Peptide inhibitor modified magnetic particles for pepsin separation. Filuszová M, Kucerová Z, Tichá M J Sep Sci. 2009 Jun;32(12):2017-21 -  
2009 TRAIL-induced apoptosis of HL60 leukemia cells: two distinct phenotypes of acquired TRAIL resistance that are accompanied with resistance to TNFα but not to idarubicin and cytarabine. Klener P Jr, Leahomschi S, Molinsky J, Simonova T, Necas E, Gasova Z, Cermak J, Dolezalova L, Andera L, Zivny J Blood Cells Mol Dis. 2009 Jan-Feb;42(1):77-84 -  
2009 The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering. Pytlík R, Stehlík D, Soukup T, Kalbácová M, Rypácek F, Trc T, Mulinková K, Michnová P, Kideryová L, Zivný J, Klener P Jr, Veselá R, Trnený M, Klener P Biomaterials. 2009 Jul;30(20):3415-27 -  
2009 Dominant renin gene mutations associated with early-onset hyperuricemia, anemia, and chronic kidney failure. Zivná M, Hůlková H, Matignon M, Hodanová K, Vylet'al P, Kalbácová M, Baresová V, Sikora J, Blazková H, Zivný J, Ivánek R, Stránecký V, Sovová J, Claes K, Lerut E, Fryns JP, Hart PS, Hart TC, Adams JN, Pawtowski A, Clemessy M, Gasc JM, Gübler MC, Antignac C, Elleder M, Kapp K, Grimbert P, Bleyer AJ, Kmoch S Am J Hum Genet. 2009 Aug;85(2):204-13 -  
2009 Identification of molecular targets for selective elimination of TRAIL-resistant leukemia cells. From spots to in vitro assays using TOP15 charts. Petrak J, Toman O, Simonova T, Halada P, Cmejla R, Klener P, Zivny J Proteomics. 2009 Nov;9(22):5006-15 -  
2010 Clinical and molecular characterization of a family with a dominant renin gene mutation and response to treatment with fludrocortisone. Bleyer AJ, Zivná M, Hulková H, Hodanová K, Vyletal P, Sikora J, Zivný J, Sovová J, Hart TC, Adams JN, Elleder M, Kapp K, Haws R, Cornell LD, Kmoch S, Hart PS Clin Nephrol. 2010 Dec;74(6):411-22 1.373  
2010 More on clinical renal genetics. Grünfeld JP, Hwu W, Chien Y, Lee N, Chiang S, Dobrovolny R, Huang A, Yeh H, Chao M, Lin S, Kitagawa T, Desnick R, Hsu L, Van Keimpema L, Nevens F, Vanslembrouck R, Van Oijen G, Hoffmann A, Dekker H, De Man R, Drenth J, Alamovitch S, Plaisier E, Favrole P, Prost C, Chen Z, Van Agrmael T, Marro B, Ronco P, Zivna M, Hulkova H, Matignon M, Hodanova K, Vylet'al P, Kalbacova M, Baresova V, Sikora J, Blazkova H, Zivny J, Ivanek R, Stranecky V, Sovova J, Claes K, Lerut E, Fryns J, Hart P, Hart T, Adams J, Pawtowski A, Clemessy M, Gasc J, Gubler M, Antignac C, Elleder M, Kapp K, Grimbert P, Bleyer A, Kmoch S, Brown E, Schlöndorff J, Becker Dj, Tsukaguchi H, Uschinski A, Higgs H, Henderson J, Pollak M. Clin J Am Soc Nephrol. 2010 Apr;5(4):563-7 -  
2010 The role of apoptosis in cancer development and treatment: focusing on the development and treatment of hematologic malignancies. Zivny J, Klener P Jr, Pytlik R, Andera L Curr Pharm Des. 2010 Jan;16(1):11-33 -  
2010 Hepcidin downregulation by repeated bleeding is not mediated by soluble hemojuvelin. Krijt J, Fujikura Y, Sefc L, Vokurka M, Hlobenová T, Necas E Physiol Res. 2010;59(1):53-9 1.430  
2010 Effect of erythropoietin on hepcidin expression in hemojuvelin-mutant mice. Krijt J, Jonásová A, Neuwirtová R, Necas E Blood Cells Mol Dis. 2010 Apr 15;44(4):257-61 2.901  
2010 Hepcidin expression in adipose tissue increases during cardiac surgery. Vokurka M, Lacinová Z, Kremen J, Kopecký P, Bláha J, Pelinková K, Haluzík M, Necas E Physiol Res. 2010;59(3):393-400 1.430  
2010 Study of urinary proteomes in Anderson-Fabry disease. Vojtová L, Zima T, Tesař V, Michalová J, Přikryl P, Dostálová G, Linhart A Ren Fail. 2010;32(10):1202-9 0.840  
2010 Stochastic model show how cochlear implants process azimuth in real auditory space. Drápal M, Maršálek P Chinese Journal of Physiology 2010, 53,6,s.1-8 0.655  
2010 Iminodiacetic acid-modified magnetic poly(2-hydroxyethyl methacrylate)-based microspheres for phosphopeptide enrichment. Novotna L, Emmerova T, Horak D, Kucerova Z, Ticha M J Chromatogr A. 2010 Dec 17;1217(51):8032-40 4.101  
2010 Restoration of Guyton´s diagram for regulation of the circulation as a basis for quantitative physiological model development. Kofránek J, Rusz J Physiol Res. 2010;59(6):897-908 1.430  
2010 Size exclusion chromatography as a tool for evaluation of fragmentation pattern of gastric mucins under non-degrading conditions. Kučerová Z, Muselová H, Miarková E, Tichá M Prague Med Rep. 2010;111(3):200-6 -  
2010 Expression of CD66 in non-Hodgkin lymphomas and multiple myeloma. Josef K, Heidi M, Robert P, Pavel K, Marek T Eur J Haematol. 2010 Dec;85(6):496-501 2.345  
2010 A sudden increase in partial pressure snd-tidal karbon dioxide (PETCO2) at the moment of return of spontaneous circulation Pokorná M;Nečas E;Kratochvíl J;Skřipský R;Andrlík M;Franěk O J Emerg Med 2010; 35(5): 614-21 1.265  
2010 The role of PU.1 and GATA-1 transcription factors during normal and leukemogenic hematopoiesis. Burda P, Laslo P, Stopka T Leukemia. 2010 Jul;24(7):1249-57 8.296  
2010 GATA-1 directly regulates p21 gene expression during erythroid differentiation. Papetti M, Wontakal SN, Stopka T, Skoultchi AI Cell Cycle. 2010 Dec 14;9(10):1972-80 4.087  
2010 Human MRCKalpha is regulated by cellular iron levels and interferes with transferrin iron uptake. Cmejla R, Ptackova P, Petrak J, Savvulidi F, Cerny J, Sebesta O, Vyoral D Biochem Biophys Res Commun. 2010 Apr 30;395(2):163-7 2.548  
2010 Adenosine A(1), A(2a), A(2b), and A(3) receptors in hematopoiesis. 1. Expression of receptor mRNA in four mouse hematopoietic precursor cells. Streitová D, Sefc L, Savvulidi F, Pospísil M, Holá J, Hofer M Physiol Res. 2010;59(1):133-7 1.430  
2011 Tyrosine 87 is vital for the activity of human protein arginine methyltransferase 3 (PRMT3). Handrkova H, Petrak J, Halada P, Pospisilova D, Cmejla R Biochim Biophys Acta. 2011 Feb;1814(2):277-82 2.480  
2011 Myocardial morphological characteristics and proarrhythmic substrate in the rat model of heart failure due to chronic volume overload. Benes J Jr, Melenovsky V, Skaroupkova P, Pospisilova J, Petrak J, Cervenka L, Sedmera D Anat Rec (Hoboken). 2011 Jan;294(1):102-11 1.490  
2011 The Atlas of Physiology and Pathophysiology: Web-based multimedia enabled interactive simulations. Kofranek J, Matousek S, Rusz J, Stodulka P, Privitzer P, Matejak M, Tribula M Comput Methods Programs Biomed. 2011 Jan 11. [Epub ahead of print] 1.144  




Kapitola v monografii:

Rok vydání Název článku Autoři Bibliografické údaje Poznámky
2007 Metabolismus a regulace zeleza v organismu ve vztahu k patogenezi aterosklerozy Vokurka M ATHEROSKLEROSA 2007 diagnostika, lecba, prevence v detskem i dospelem veku, 12.-14.9. 2007, Praha, Ceska republika, ISBN 978-80-254-0238-2  




Dizertační práce:

Rok dokončení Název (téma) Autor Popis Poznámky
2006 Využití chelatovaných iontů kobaltu pro imobilizaci a separaci proteinů. Zatloukalová, Eva, Mgr - Obhájená dizertace
2006 Analysis of TRAIL- induced apoptosis in acute myeloid leukemia cells Klener Pavel jr. - Obhajoba PhD
2010 Separace hepcidinu na magnetických sorbetech s následnou analýzou pomocí MALDI-TOF MS Vávrová J - Diplomová práce
2010 Regulace genové exprese v průběhu normální a leukemické diferenciace 2010, s. 250 Stopka T - Habilitační práce




Postery:

Rok Název Autoři Popis Poznámky
2007 Gene expression of hepcidin in adipose tissue increases during surgery in humans and after lipopolysaccharide administration in mice Vokurka M, Lacinova Z, Kremen J, Krijt J, Krajickova J, Blaha J, Pelinkova K, Haluzik M, Necas E EIC/ European Iron Club, Kings College London, Guys Campus, 13th-15th Septembet 2007, London, UK Ne Poster: P61 str. 113  
2007 Lipopolysaccharide - induced hypoferremia is not influenced by hemojuvelin gene knockout Krijt J, Vokurka M, Necas E EIC/ European Iron Club, Kings College London, Guys Campus, 13th-15th Septembet 2007, London, UK (Poster: P25, str. 77)