Microscope

Motorization

• nosepiece
• condensers
• BP filter turret
• Z - drive
• XY stage (PRIOR ProscanIII)
• analyzer
• epifluorescence shutter
• light-path (5 ports)
• diascopic illumination (C1si)

Objectives

• PlanApo VC 60x N.A. 1.40 (oil)*
• Plan Fluor 40x N.A.1.30 (oil)
• Plan Fluor 10x N.A. 0.30
• PlanApo VC 20x N.A. 0.75*
• PlanApo 40x N.A. 0.95
• Apo TIRF 60x N.A. 1.49*

* DIC available

Condensers

• LWD Abbe
• LWD DIC N2 (Nomarski)
• Bertrand lens for Ph ring alignment
• PlanApo VC 20x N.A. 0.75
• PlanApo 40x N.A. 0.95
• Apo TIRF 60x N.A. 1.49

FilterCubes (WF fluorescence)

• UV-2A Ex.330-380/ DM 400/Em.420L
• FITC Ex.465-495/DM505/Em.515-555
• TRITC Ex.528-553/DM565/Em.578-633
• PlanApo VC 20x N.A. 0.75
• PlanApo 40x N.A. 0.95
• Apo TIRF 60x N.A. 1.49

CCD camera

• Nikon DS-Qi1Mc

WF excitation source

• EXFO Xcite 120PC

Laser scanning confocal system

Laser lines

• 405nm diode (AOTF)
• 488nm line Ar (AOTF)*
• 514nm line Ar (AOTF)*
• 543nm HeNe (AOTF)
• 633nm HeNe (ND4+ND8)
  
  *only one of the lines can be used in one Fr series

Scannnig head DM

• 488/543/633nm
• 405/488/543nm
• 20/80 (R/T)
• 10/90 (R/T)

PMT filter cubes

• Set 1: 515+/-15nm, 590+/-15nm, 650nmLP
• Set 1: 450+/-15nm, 515+/-15nm, 600nmLP

Spectral Detection

• 32channels in 400-650nm range
• channels width 2.5nm/5nm/10nm
• linear anmixing algoritm based on single value decomposition method

Perfect Focus System

• Nikon 2nd generation with offset. Non suitable for plastic dishes and PH objectives
  

LiveScan Swept field confocal system (SFC NIKON - Prairie inc.)

Laser lines

• 488nm (Sapphire 488-50
• 561nm (Jive-50)
• 643nm (RCL-035-643)

Camera

• Photometrics Cascade 512II

Scanning modes

• pinhole modes (up to 100fps)
• slit mode (up to 250 fps)

Software

• EZ-C1 v.391 (C1 cofocal aquision)
• NIS elements v. 4.13 (SFC, WF, BF)
• Huygenes v.4 (deconvolution, visualization)
• FiJi (image analysis and adjustments)