Microscope - DMi8
Motorization
- nosepiece
- condensers
- BP filter turret
- Z - drive
- Super galvo Z drive
- XY stage
- analyzer
- epifluorescence shutter
- light-path
- diascopic illumination
Objectives
* DIC available
CCD camera
WF excitation source
FilterCubes (WF fluorescence)
- DAPI Ex.BP360/40-DM400-Em.470/40
- GFP (for AlexaFluor488) Ex.BP470/40-DM500-Em.BP525/50
- DsRED (for AlexaFluor568) Ex.BP546/11-DM560-Em.BP605/75
- Y5 (for AlexaFluor633) Ex.BP620/60-DM660-Em.BP700/75
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Laser scanning confocal system
Laser lines
- 405nm diode/50mW DMOD Flexible
- 488nm line Ar (AOTF)*
- 458nm line Ar (AOTF)*
- 476nm line Ar (AOTF)*
- 488nm line Ar (AOTF)*
- 596nm line Ar (AOTF)*
- 514nm line Ar (AOTF)*
- 470nm-670nm 80MHz pulse continuum WLL2 **
* total combined power - 65mW
** avrg. 1.5mW/1nm
Dichroics - Excitation/Emission separation
- Acousto Optical Beam Splitter available to couple up to 8 independent laser lines simuntaneusly (separation at least 5nm). Servers as dichroic mirrors and emmision filters
Emmision spectral detection
- Dispersion of emmision signal on glass prism.
- Detectino of up to four channesl on 2xhybrid detectors and 2xclassical PMTs
- Detection range 400nm-800nm
- Transmition light detector for "confocal" DIC
Scanners
- FOV scanner for large field of view scanning up to 7fps (512x512px), 22mm diagonal FOV, up to 8192x8192px
- Resonant scanner for fast scanning at 28fps (512x512px), 13mm diagonal, up to 1024x1024px
Live cell attachments
- Adaptive Focus Control with active retaining of focus position (feedback)
- Enclosement of the system in heated box
- Compatible with OKOlab stage top incubators (6/12/24 well plates, u-slides, 35mm dishes)
- Regulation of temperature, CO2 and O2 concentration, humidifier
- Compatible with live cell super galvo fast Z-scanning
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